Drug Testing in Charlotte, North Carolina

US Drug Test Centers has more than 20,000 testing locations nationwide. We offer drug and alcohol testing in Charlotte, North Carolina for both Department of Transportation (DOT) and non-DOT companies, along with individuals. We provide affordable urine drug testing, hair follicle drug testing, breath alcohol tests, and more in Charlotte. We only work with SAMHSA-certified laboratories, and all test results are verified by a medical review officer (MRO).

Order a test online or call 866-566-0261 for more help.

Committed to Accuracy

We only work with SAMHSA-certified laboratories and have all test results confirmed by a medical review officer.

Unparalleled Convenience

With drug testing locations spread throughout Charlotte, North Carolina, you never have to go far to find us. For speedy results, we offer instant urine drug testing and on-site mobile testing.

Endless Variety

We can screen for dozens of illicit substances via hair, urine, and breath. Additionally, we have hundreds of drug test panels to choose from.

100 Testing locations in Charlotte, North Carolina

3123 N Davidson St

Charlotte North Carolina 28205

Phone: 866-566-0261

1909 J N Pease Pl

Charlotte North Carolina 28262

Phone: 866-566-0261

1914 J N Pease Pl

Charlotte North Carolina 28262

Phone: 866-566-0261

10410 Park Rd

Charlotte North Carolina 28210

Phone: 866-566-0261

2711 Randolph Rd

Charlotte North Carolina 28207

Phone: 866-566-0261


95 more location(s) available in Charlotte , North Carolina

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Local Reviews

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Review by A.S.
on November 16, 2022


US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company's needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

Frequently Asked Questions About North Carolina Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated Jun 2023

North Carolina Drug Testing FAQs

Do employers in North Carolina need to comply with mandatory state-specific rules when drug & alcohol testing?
Yes. North Carolina has specific rules for employers that wish to conduct employee drug testing.
Is a Drug-Free Workplace policy required in North Carolina?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the North Carolina’s voluntary law).
What are some compliance requirements in North Carolina?
Employers are required to use HHS/SAMHSA, or CAP approved laboratories. Employers are required to ensure that their service providers comply with all state drug testing laws. At the time of each test, the employer must give the donor a written notice of their rights under the law. On-site testing is permitted for pre-employment drug testing only. Written notice of a positive test result must be given but may be given in any manner such as in person, mail or otherwise. Everyone tested has a right to a retest and must pay all reasonable expenses; reasonable expenses mean in part actual costs of retest and chain of custody procedures.  The individual tested may only be asked to pay up to $15 of this cost.
Are employers in North Carolina able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may be able to leverage a ‘Rebuttable Presumption of Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test may also make an employee ineligible for unemployment compensation if terminated for misconduct. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Does North Carolina authorize marijuana (THC) or cannabidiol (CBD) use?
Yes. North Carolina authorizes the medical use of cannabidiol (CBD) for very limited medical conditions. The law does not address the workplace.
Can I be fired or lose a job offer in North Carolina if I fail a drug test?
In North Carolina, if you test positive on a drug test it can be grounds for termination or a rescinded job offer.

Drug Testing Stats - Charlotte, North Carolina

Drug and Alcohol Abuse Statistics from Charlotte, North Carolina

Last Updated: February 6, 2024

  • 82.70% of adults 35 and older in North Carolina died from excessive alcohol use.
  • Each year in North Carolina, 18.0 of every 100,000 residents living there's cause of death is opioid overdose.
  • Each year in North Carolina, doctors write prescriptions for opioids for 61.50% of their patients.
  • Each year in North Carolina, 22.3 of every 100,000 residents living there's cause of death is drug overdose.
  • North Carolina is 7.73% below the national death rate of death caused by substance abuse.

North Carolina’s Substance Abuse Problem

In North Carolina, an average of approximately 4,452 deaths are attributable to excessive alcohol consumption. Additionally, 2,266 people will die from a drug overdose each and every year. The opioid epidemic is present in North Carolina, as well, with 783 opioid overdose deaths annually.

The Financials of Rehab

The U.S. public expenditure on outpatient drug rehabilitation services is approximately 3.45% or $79.70 million. Rehab isn’t cheap for those who need treatment, adding up to about $56,759 per individual for inpatient treatment and $1,703 for outpatient rehabilitation.


  1. The National Survey on Drug Use and Health (NSDUH), conducted annually by the Substance Abuse and Mental Health Services Administration (SAMHSA)
  2. Provisional Multiple Cause of Death database contains mortality and population for U.S. counties.
  3. Alcohol Use in the United States: Age Groups and Demographic Characteristics

Available Collection Types

US Drug Test Centers offers the full range of collection types, including urine, hair and breath samples, each with its own advantages and applications for accurate substance screening.

Legal Drug Testing

Legal drug testing is a critical procedure often mandated by employers, law enforcement agencies, and organizations to ensure compliance with regulations and maintain safety in various settings. US Drug Test Centers can help you with your testing needds.

DOT Testing Services

DOT Testing Services are specifically designed to meet the stringent drug and alcohol testing requirements mandated by the U.S. Department of Transportation (DOT) for safety-sensitive positions in the transportation industry, ensuring compliance and public safety.

Laboratory Testing Services

Looking for nationwide laboratory testing? US Drug Test Centers has partnered with eNational Testing to offer laboratory testing such as blood titers (MMR, Varicella, Hep B), QuantiFERON (TB Blood) Testing, Mens & Womens health panels, allergy testing and much more! You can simply order a health lab test or panel online 24/7 and go in for testing the same day at over 2,700 locations nationwide.

Scheduling your preventative health laboratory testing in Charlotte, North Carolina here.

Our Drug Testing Partners