Drug Testing in Milwaukee, Wisconsin

US Drug Test Centers has more than 20,000 testing locations nationwide. We offer drug and alcohol testing in Milwaukee, Wisconsin for both Department of Transportation (DOT) and non-DOT companies, along with individuals. We provide affordable urine drug testing, hair follicle drug testing, breath alcohol tests, and more in Milwaukee. We only work with SAMHSA-certified laboratories, and all test results are verified by a medical review officer (MRO).

Order a test online or call 866-566-0261 for more help.

Committed to Accuracy

We only work with SAMHSA-certified laboratories and have all test results confirmed by a medical review officer.

Unparalleled Convenience

With drug testing locations spread throughout Milwaukee, Wisconsin, you never have to go far to find us. For speedy results, we offer instant urine drug testing and on-site mobile testing.

Endless Variety

We can screen for dozens of illicit substances via hair, urine, and breath. Additionally, we have hundreds of drug test panels to choose from.

37 Testing locations in Milwaukee, Wisconsin

1311 N 6Th St

Milwaukee Wisconsin 53212

Phone: 866-566-0261

638 N 18Th St

Milwaukee Wisconsin 53233

Phone: 866-566-0261

215 N 35th St

Milwaukee Wisconsin 53208

Phone: 866-566-0261

875 W Layton Ave

Milwaukee Wisconsin 53221

Phone: 866-566-0261

734 N Jackson St

Milwaukee Wisconsin 53202

Phone: 866-566-0261


32 more location(s) available in Milwaukee , Wisconsin

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Local Reviews

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Review by R.J.
on November 22, 2023

"Staff makes sure you are aware of how to keep your company compliant with laws and have helped me through multiple processes that were new to me."

Review by J.S.
on August 19, 2023

"We chose you because of availability of test centers being close by. But we haven’t been able to utilize any in our area because of the tests being requested. Inconvenient and costs us money to miss work"

Review by H.B.
on June 14, 2023


Review by B.M.
on January 24, 2023

"Very fast and great personalities!!"

Review by M.N.
on January 9, 2023

"Simple and easy"

Review by J.J.
on November 22, 2022

"They are very helpful"

Review by S.K.
on October 25, 2022

"Easy to sign up"

US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company's needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

Frequently Asked Questions About Wisconsin Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated May 2023

Wisconsin Drug Testing FAQs

Do employers in Wisconsin need to comply with mandatory state-specific rules when drug & alcohol testing?
No. Wisconsin does not have any mandatory drug testing statutes or regulations, except for Public Works employees and contractors. However, drug testing is recommended.
Is a Drug-Free Workplace policy required in Wisconsin?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the Wisconsin’s voluntary law).
Are employers in Wisconsin able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may be able to leverage an ‘Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test or refusal to test may also make an employee ineligible for unemployment compensation if terminated for misconduct. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Does Wisconsin authorize marijuana (THC) or cannabidiol (CBD) use?

Yes. Wisconsin authorizes the medical use of cannabidiol (CBD) for very limited medical conditions. The law does not address the workplace.

Can I be fired or lose a job offer in Wisconsin if I fail a drug test?
In Wisconsin, if you test positive on a drug test it can be grounds for termination or a rescinded job offer.
What are the medical and recreational marijuana-related rules in Wisconsin?
In Wisconsin, marijuana use, both recreational and medical use are not considered legal in the workplace and only low THC products are allowed.

Drug Testing Stats - Milwaukee, Wisconsin

Drug and Alcohol Abuse Statistics from Milwaukee, Wisconsin

Last Updated: February 6, 2024

  • Studies show that 1.7 adults in Wisconsin binge drink on more than 1 day on monthly basis.
  • As a result of excessive alcohol use, in Wisconsin in 2010 taxpayers spent approximately $4.453 billion towards alcohol.
  • In Wisconsin, the death rate per 100,000 residents due to opioid overdose was 15.0
  • In Wisconsin, 6,216 people who used a needle for drug use contracted HIV/AIDS in a year.
  • Each year in Wisconsin, 21.1 of every 100,000 residents living there's cause of death is drug overdose.

The Opioid Epidemic

In Wisconsin, 846 people die from an opioid overdose every single year. 45.80% of doctors write opioid prescriptions for their patients. Opioid use often leads to heroin use, which can happen via injection. And, interestingly, 27,900 cases of hepatitis C are attributed to intravenous drug use.

The Cost to Rehabilitate

Rehab facilities in Wisconsin are fighting to stop the abuse of opioids and other drugs. There are 327 active clinics in the state, serving 25,574 individuals every year. The U.S. public’s total spend on outpatient services is 1.77% or $40.89 million, with the cost to individuals in inpatient rehab a whopping $56,625.


  1. The National Survey on Drug Use and Health (NSDUH), conducted annually by the Substance Abuse and Mental Health Services Administration (SAMHSA)
  2. Provisional Multiple Cause of Death database contains mortality and population for U.S. counties.
  3. Alcohol Use in the United States: Age Groups and Demographic Characteristics

Available Collection Types

US Drug Test Centers offers the full range of collection types, including urine, hair and breath samples, each with its own advantages and applications for accurate substance screening.

Legal Drug Testing

Legal drug testing is a critical procedure often mandated by employers, law enforcement agencies, and organizations to ensure compliance with regulations and maintain safety in various settings. US Drug Test Centers can help you with your testing needds.

DOT Testing Services

DOT Testing Services are specifically designed to meet the stringent drug and alcohol testing requirements mandated by the U.S. Department of Transportation (DOT) for safety-sensitive positions in the transportation industry, ensuring compliance and public safety.

Laboratory Testing Services

Looking for nationwide laboratory testing? US Drug Test Centers has partnered with eNational Testing to offer laboratory testing such as blood titers (MMR, Varicella, Hep B), QuantiFERON (TB Blood) Testing, Mens & Womens health panels, allergy testing and much more! You can simply order a health lab test or panel online 24/7 and go in for testing the same day at over 2,700 locations nationwide.

Scheduling your preventative health laboratory testing in Milwaukee, Wisconsin here.

Our Drug Testing Partners