Orlando, Florida Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


79 Testing locations in Orlando, Florida

4382 L.b. Mcleod Road

Orlando Florida 32811

Phone: 866-566-0261

8989 South Orange Avenue

Orlando Florida 32824

Phone: 866-566-0261

9685 Lake Nona Village Place

Orlando Florida 32827

Phone: 866-566-0261

7121 Grand National Dr Ste 103

Orlando Florida 32819

Phone: 866-566-0261

9753 South Orange Blossom Trail

Orlando Florida 32837

Phone: 866-566-0261

3010 Hunter's Creek Boulevard

Orlando Florida 32837

Phone: 866-566-0261

2075 Town Center Boulevard

Orlando Florida 32837

Phone: 866-566-0261

709 E. Michigan Street

Orlando Florida 32806

Phone: 866-566-0261

1803 Park Center Drive

Orlando Florida 32835

Phone: 866-566-0261

709 E Michigan St

Orlando Florida 32806

Phone: 866-566-0261

7751 Kingspointe Parkway

Orlando Florida 32819

Phone: 866-566-0261

2323 S. Orange Ave

Orlando Florida 32806

Phone: 866-566-0261

2555 S. Kirkman Rd

Orlando Florida 32811

Phone: 866-566-0261

7751 Kings Pointe Parkway

Orlando Florida 32819

Phone: 866-566-0261

4301 E Colonial Dr

Orlando Florida 32803

Phone: 866-566-0261

2555 S Kirkman Rd

Orlando Florida 32811

Phone: 866-566-0261

2323 South Orange Ave

Orlando Florida 32806

Phone: 866-566-0261

6490 Hazeltine National Drive

Orlando Florida 32822

Phone: 866-566-0261

701 E Michigan St

Orlando Florida 32806

Phone: 866-566-0261

6965 Piazza Grande Avenue

Orlando Florida 32835

Phone: 866-566-0261

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Phone: 866-566-0261

Drug Testing in Orlando, Florida

US Drug Test Centers offers all types of drug and alcohol tests in Orlando. We provide over 20 urine, hair, and breath alcohol tests. You can learn of negative results the same day with some of these tests. We even have a DOT-compliant test that normally delivers negative results in 24 to 48 hours.

Our Healthcare Professional Panel is also available in Orlando. This panel tests for specific, highly addictive prescription medications that are readily accessible to workers in this field. This is a urine test which determines the presence or absence of the most abused prescription drugs or their metabolites. Our Healthcare Professional Panel is also an excellent standard for allied health students.

US Drug Test Centers has over 35 locations in and near Orlando. Our testing locations require a drug test order form / donor pass and your photo ID. We can provide you with a drug test order form / donor pass within minutes of calling our office at 866-566-0261 or by placing your order online. US Drug Test Centers do not accept payment on site. Most of our testing locations accept walk-ins or same day appointments to minimize wait times for your drug test Orlando FL.

About Orlando, Florida

Orlando is located in central Florida. It is the county seat of Orange County. Orlando has a population over 230,000. The principal cities in the Orlando metro area are Orlando, Kissimmee, and Sanford. Millions of people travel to Orlando each year to visit Walt Disney World, SeaWorld Orlando, and Universal Orlando.

Who Can Benefit from Drug or Alcohol Testing from US Drug Test Centers?

  • Wellness Programs required drug and alcohol testing
  • Rehab Facilities and Healthcare Facilities required drug and alcohol testing
  • Family Courts required drug and alcohol testing
  • Law Enforcement Agencies required drug and alcohol testing
  • Schools and Parents required drug and alcohol testing
  • Last Chance Agreements required drug and alcohol testing
  • Adoptions required drug and alcohol testing
  • DUI and DWI Offenders required drug and alcohol testing
  • Probation and Parole required drug and alcohol testing
  • Child Custody cases required drug and alcohol testing
  • Child Protection cases required drug and alcohol testing

Legally Mandated Drug Tests in Orlando

US Drug Test Centers can perform drug tests required by probation or court orders. This service can be provided on a daily basis or as necessary. Our drug testing specialists are committed to the highest standards. This ensures properly administered tests and accurate results. 

Employee and pre-employment drug testing in Orlando

A drug-free facility benefits everyone in the workplace. Committing to a drug-free policy can also reduce your exposure to liability related to drug or alcohol related incidents. US Drug Test Centers can help your company implement and maintain a drug-free environment with our complete range of drug testing services.

We offer extremely fast results for DOT and non-DOT drug tests – 93% of our negative drug test results are reported in 24 to 48 hours. Instant testing for non-DOT programs are also available with same day negative results.

Personal Drug Testing in Orlando

Our drug testing facilities in Orlando can perform immediate drug tests for individuals which may be required in the following situations: by court order, probation, divorce hearings, custody hearings, and in other legal and employment circumstances. We have over 35 drug testing centers in Orlando for your convenience. These drug tests are done the same day that they are requested and the results are usually delivered quickly.

Order your drug and alcohol test Orlando Florida today.

DOT Compliant Drug Testing

If you are a DOT regulated company based in Orlando, Florida then you must have a DOT compliant drug testing program. A drug testing program administered and maintained by US Drug Test Centers in Orlando can pass DOT audits at any time. We are qualified to offer all key components of a DOT compliant drug test.

Online Training for Businesses in Orlando, Florida

US Drug Test Centers offer a variety of affordable online training programs for your supervisors. There are DOT and non-DOT courses. Your supervisors may have already taken online drug and alcohol training. These courses are revised and updated annually as new information becomes available. It is recommended that supervisor refresher training is completed every 12 to 18 months.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.


Frequently Asked Questions About Florida Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated Dec 2022

Florida Drug Testing FAQs

Do employers in Florida need to comply with mandatory state-specific rules when drug & alcohol testing?
No. Florida does not have any mandatory drug testing statutes or regulations but it is recommended.
Is a Drug-Free Workplace policy required in Florida?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the Florida’s voluntary law).
Are employers in Florida able to leverage their drug screening program to reduce their Worker’s Compensation Insurance costs?
Yes. Florida provides employers with the ability to voluntarily participate in a Drug-Free Workplace Program to qualify for workers’ compensation insurance premium reductions. Employers wishing to leverage these benefits must voluntarily comply with very detailed program rules.
Are employers in Florida able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may leverage an ‘Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
What are some nuances of the voluntary Drug-Free Workplace Program requirements in Florida?
Employers must have a policy that follows the rules defined by the Florida Department of Health. Employees and applicants must be provided notice with the list of drugs to be tested and their related street names. Construction contractors in public works projects must comply with the states Drug-Free Workplace Program.
Does Florida authorize marijuana (THC) or cannabidiol (CBD) use?
Yes. Florida permits the medical use of marijuana for patients with limited medical conditions.  The laws do not specifically impact the workplace.

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