Louisville, Kentucky Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


44 Testing locations in Louisville, Kentucky

820 South 6th Street

Louisville Kentucky 40203

Phone: 866-566-0261

2307 Bardstown Rd

Louisville Kentucky 40205

Phone: 866-566-0261

4003 Kresge Way

Louisville Kentucky 40207

Phone: 866-566-0261

7092 Distribution Drive

Louisville Kentucky 40258

Phone: 866-566-0261

3303 Fern Valley Rd

Louisville Kentucky 40213

Phone: 866-566-0261

11630 Commonwealth Dr

Louisville Kentucky 40299

Phone: 866-566-0261

228 1/2 Breckenridge Lane

Louisville Kentucky 40207

Phone: 866-566-0261

228 Breckenridge Lane

Louisville Kentucky 40207

Phone: 866-566-0261

300 High Rise Drive

Louisville Kentucky 40213

Phone: 866-566-0261

7702 Preston Highway

Louisville Kentucky 40219

Phone: 866-566-0261

12711 Towne Park Way

Louisville Kentucky 40243

Phone: 866-566-0261

4010 Dupont Cir

Louisville Kentucky 40207

Phone: 866-566-0261

1230 South Hurstbourne Parkway

Louisville Kentucky 40222

Phone: 866-566-0261

7400 New Lagrange Rd

Louisville Kentucky 40222

Phone: 866-566-0261

10110 Dixie Highway

Louisville Kentucky 40272

Phone: 866-566-0261

1321 Herr Lane

Louisville Kentucky 40222

Phone: 866-566-0261

539 South Fourth Street

Louisville Kentucky 40295

Phone: 866-566-0261

200 Abraham Flexner Way

Louisville Kentucky 40202

Phone: 866-566-0261

210 East Gray Street

Louisville Kentucky 40202

Phone: 866-566-0261

4420 Dixie Highway

Louisville Kentucky 40216

Phone: 866-566-0261

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Phone: 866-566-0261

Drug Testing in Louisville, Kentucky

US Drug Test Centers offers all types of drug and alcohol tests in Louisville. We provide over 20 urine, hair, and breath alcohol tests. You can learn of negative results the same day with some of these tests. We even have a DOT-compliant test that normally delivers negative results in 24 to 48 hours for your drug test Louisville KY.

US Drug Test Centers in Louisville can also run alcohol specific tests. These include a variety of urine tests, hair tests, and breath alcohol testing. The EtG hair test measures the average ingestion of Ethyl Alcohol over a three month period. While this does not detect current impairment it can indicate a behavioral issue.

US Drug Test Centers has over 20 locations in and near Kentucky. Our testing locations require a drug test order form / donor pass and your photo ID. We can provide you with a drug test order form / donor pass within minutes of calling our office at 866-566-0261 or by placing your order online. US Drug Test Centers do not accept payment on site. Most of our testing locations accept walk-ins or same day appointments to minimize wait times for drug testing Louisville KY.

About Louisville, Kentucky

Louisville was founded in 1778 and is one of the oldest cities west of the Appalachian Mountains. Louisville was the county seat of Jefferson County until the two governments merged in 2003. The largest city of Kentucky has a population of nearly 800,000 people. Given Louisville's centralized location, it is approximately one day's road trip to 60% of the cities in the 48 contiguous states in the US.

Legally Mandated Drug Tests in Louisville

US Drug Test Centers can perform drug tests required by probation or court orders. This service can be provided on a daily basis or as necessary. Our drug testing specialists are committed to the highest standards. This ensures properly administered tests and accurate results.

Employee and pre-employment drug testing in Louisville

A drug-free facility benefits everyone in the workplace. Committing to a drug-free policy can also reduce your exposure to liability related to drug or alcohol related incidents. US Drug Test Centers can help your company implement and maintain a drug-free environment with our complete range of drug testing services.

We offer extremely fast results for DOT and non-DOT drug tests – 93% of our negative drug test results are reported in 24 to 48 hours. Instant testing for non-DOT programs are also available with same day negative results.

Personal Drug Testing in Louisville

Our drug testing facilities in Louisville can perform immediate drug tests for individuals which may be required in the following situations: by court order, probation, divorce hearings, custody hearings, and in other legal and employment circumstances. We have over 20 drug testing centers in Louisville for your convenience. These drug tests are done the same day that they are requested and the results are usually delivered quickly.

DOT Compliant Drug Testing

If you are a DOT regulated company based in Louisville, Kentucky then you must have a DOT compliant drug testing program. A drug testing program administered and maintained by US Drug Test Centers in Louisville can pass DOT audits at any time. We are qualified to offer all key components of a DOT compliant drug test.

Online Training for Businesses in Louisville, Kentucky

US Drug Test Centers offer a variety of affordable online training programs for your supervisors. There are DOT and non-DOT courses. Your supervisors may have already taken online drug and alcohol training. These courses are revised and updated annually as new information becomes available. It is recommended that supervisor refresher training is completed every 12 to 18 months.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.


Frequently Asked Questions About Kentucky Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated Dec 2022

Kentucky Drug Testing FAQs

Do employers in Kentucky need to comply with mandatory state-specific rules when drug & alcohol testing?
No. Kentucky does not have any mandatory drug testing statutes or regulations but it is recommended.
Is a Drug-Free Workplace policy required in Kentucky?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the Kentucky’s voluntary law).
Are employers in Kentucky able to leverage their drug screening program to reduce their Worker’s Compensation Insurance costs?
Yes. Kentucky provides employers with the ability to voluntarily participate in a Drug-Free Workplace Program to qualify for workers’ compensation insurance premium reductions. Employers wishing to leverage these benefits must voluntarily comply with very detailed program rules.
Are employers in Kentucky able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may leverage an ‘Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
What are some nuances of the voluntary Drug-Free Workplace Program requirements in Kentucky?
Kentucky’s voluntary law requires employers to create a drug testing policy. Employers must post a notice that their company is a drug-free workplace and must provide a copy of the policy to each employee or applicant. The adoption of an employee assistance program (EAP) is also required.
Does Kentucky authorize marijuana (THC) or cannabidiol (CBD) use?
Yes. Kentucky authorizes the medical use of cannabidiol for very specific medical conditions. The law does not address the workplace.

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