Kentucky Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


Kentucky, the bluegrass state has been hit by a growing epidemic. Heroin has been an issue in Kentucky; recently Fentanyl has posed even larger problems. Classified as a schedule II narcotic, Fentanyl is 50 times more addictive than heroin. Cities in Kentucky including Louisville and Lexington have been it the hardest. Kentucky is in the top ten states for opioid addiction and related overdoses. High rates of drug use hurt the entire community. US Drug Test Center offers online drug test information and resources for you or someone you know.

Kentucky employers have the right to enforce a drug free workplace to ensure productivity and safety. Employers who wish to participate in workers compensation programs may test employees under reasonable suspicion of use of drugs or alcohol, following an accident that took place at the workplace, as a follow up to a rehabilitation program or at random. Employers may also request an alcohol test upon initial employment or for the reasons listed previously. US Drug Test Centers makes this easy. In some situations, same day testing may be available for employers and individuals seeking testing.

Finding a Drug Test Center Near You

With 120 counties in Kentucky and over 300 locations, there is a US Drug Test Center near you. Jefferson County including Louisville with 44 locations (one conveniently located off S Fourth in the heart of downtown and in zip codes 40218, 40203, 40213, 40217, 40218, 40223, 40291, and 40214), 25 in Lexington (off Nicholasville Rd. behind the University of Kentucky Arboretum) and even Somerset in Pulaski County where we have 5 locations. Other counties and cities with a location include; Kenton, Boone, and Warren and Hardin.

Same day drug testing available for employers and individuals in all areas of Kentucky. We can arrange a single test or a testing program, depending on your needs. Call early in the day for same day drug testing services. All drug tests can also be ordered with one quick phone call – Call 866-566-0261.

US Drug Test Centers

US Drug Test Centers is the complete, drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty.We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing.

We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. At USDTC, we can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings.

Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program.

We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

Frequently Asked Questions About Kentucky Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated June 2019

Kentucky Drug Testing FAQs

No. Kentucky does not have any mandatory drug testing statutes or regulations but it is recommended.
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the Kentucky’s voluntary law).
Yes. Kentucky provides employers with the ability to voluntarily participate in a Drug-Free Workplace Program to qualify for workers’ compensation insurance premium reductions. Employers wishing to leverage these benefits must voluntarily comply with very detailed program rules.
Yes. Employers may leverage an ‘Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Kentucky’s voluntary law requires employers to create a drug testing policy. Employers must post a notice that their company is a drug-free workplace and must provide a copy of the policy to each employee or applicant. The adoption of an employee assistance program (EAP) is also required.
Yes. Kentucky authorizes the medical use of cannabidiol for very specific medical conditions. The law does not address the workplace.

320 Testing locations in Kentucky

801 Tennessee Road

Phone: 866-566-0261

336 29th Street Suite 204

Phone: 866-566-0261

4360 13th Street

Phone: 866-566-0261

4305 New Shepherdsville Road

Phone: 866-566-0261

919 Chambers Boulevard

Phone: 866-566-0261

83 Wellness Way Lane, Ste B

Phone: 866-566-0261

1110 Wilkinson Trace

Phone: 866-566-0261

1300 Andrea Suite 100

Phone: 866-566-0261

4863b Scottsville Rd

Phone: 866-566-0261

6807 Louisville Road

Phone: 866-566-0261

1111 Lovers Ln Ste 101

Phone: 866-566-0261

5575 Scottsville Road

Phone: 866-566-0261

1225 Fairway St # St100

Phone: 866-566-0261

2978 State Route 2551

Phone: 866-566-0261

254 Main Street

Phone: 866-566-0261

1700 Old Lebanon Road

Phone: 866-566-0261

95 Bryan Boulevard

Phone: 866-566-0261

1400 Cumberland Falls Highway

Phone: 866-566-0261

1321 Cumberland Falls Highway

Phone: 866-566-0261

2327 Buttermilk Crossing

Phone: 866-566-0261

500 Thomas More Pkwy #8

Phone: 866-566-0261

2616 Legends Way Ste B

Phone: 866-566-0261

200 Corporate Drive

Phone: 866-566-0261

217 S. 3rd Street

Phone: 866-566-0261

380 Whirl-a-way Dr

Phone: 866-566-0261

200 Medical Village Drive

Phone: 866-566-0261

200 Medical Village Dr

Phone: 866-566-0261

903 W Stockton St

Phone: 866-566-0261

1825 Airport Exchange Blvd

Phone: 866-566-0261

650 Sprucewood Ln

Phone: 866-566-0261

1107 Bellefonte Rd

Phone: 866-566-0261

7220 Burlington Pike

Phone: 866-566-0261

7370 Turfway Rd Ste 170

Phone: 866-566-0261

10095 Investment Way

Phone: 866-566-0261

859 E Main St Ste 2c

Phone: 866-566-0261

101 Medical Heights Dr Ste G

Phone: 866-566-0261

119 Memorial Drive

Phone: 866-566-0261

208 South Main Street

Phone: 866-566-0261

1100 Brookhaven Rd

Phone: 866-566-0261

2000 Holiday Lane

Phone: 866-566-0261

1150 Lexington Road

Phone: 866-566-0261

1140 Lexington Rd Bldg A

Phone: 866-566-0261

401 Outlet Center Drive

Phone: 866-566-0261

1502 Old Oxford Cir

Phone: 866-566-0261

411 S L Rogers Wells Blvd

Phone: 866-566-0261

609 No Carol Malone Blvd

Phone: 866-566-0261

440 Hopkinsville Street

Phone: 866-566-0261

120 Professional Ln Ste 101

Phone: 866-566-0261

123 East Central Street

Phone: 866-566-0261

572 Village Lane

Phone: 866-566-0261

101 Town & Country Lane

Phone: 866-566-0261

145 Citizens Ln Ste B

Phone: 866-566-0261

104 North Elm Street

Phone: 866-566-0261

110 Third Street Suite 100

Phone: 866-566-0261

1305 North Elm Street

Phone: 866-566-0261

2003 S. 7th Street

Phone: 866-566-0261

1115 West Cumberland Avenue

Phone: 866-566-0261

10216 Taylorsville Rd Ste 750

Phone: 866-566-0261

7786 Us Highway 27n

Phone: 866-566-0261

110 Pleasant Retreat Drive

Phone: 866-566-0261

312 West High Street

Phone: 866-566-0261

1895 Elizabethtown Road

Phone: 866-566-0261

3439 Buckhorn Dr # 130

Phone: 866-566-0261

1740 Nicholasville Rd

Phone: 866-566-0261

1500 Leestown Road

Phone: 866-566-0261

1055 Dove Run Road

Phone: 866-566-0261

2424 Sir Barton Way

Phone: 866-566-0261

155 Prosperous Place

Phone: 866-566-0261

211 Fountain Court

Phone: 866-566-0261

3475 Richmond Road

Phone: 866-566-0261

1000 Monarch St Ste 180

Phone: 866-566-0261

3475 Richmond Rd #260

Phone: 866-566-0261

3061 Fieldstone Way

Phone: 866-566-0261

1401 Harrisburg Rd. Ste# C-45

Phone: 866-566-0261

141 N Eagle Creek Dr Ste 103

Phone: 866-566-0261

424 Lewis Hargett Circle

Phone: 866-566-0261

160 North Eagle Creek Drive

Phone: 866-566-0261

150 N Eagle Creek Dr

Phone: 866-566-0261

3174 Custer Dr., Ste. 100

Phone: 866-566-0261

187 Wolford Avenue

Phone: 866-566-0261

511 Middleburg St

Phone: 866-566-0261

300 Dog Patch Trading Center

Phone: 866-566-0261

1364 S Laurel Rd

Phone: 866-566-0261

85 South Laurel Road

Phone: 866-566-0261

360 Spring Street

Phone: 866-566-0261

412 N. Lock Avenue

Phone: 866-566-0261

2583 Kentucky 644

Phone: 866-566-0261

820 South 6th Street

Phone: 866-566-0261

7092 Distribution Drive

Phone: 866-566-0261

3303 Fern Valley Rd

Phone: 866-566-0261

11630 Commonwealth Dr

Phone: 866-566-0261

228 Breckenridge Lane, Suite B

Phone: 866-566-0261

228 1/2 Breckenridge Lane

Phone: 866-566-0261

228 Breckenridge Lane

Phone: 866-566-0261

300 High Rise Drive

Phone: 866-566-0261

7702 Preston Highway

Phone: 866-566-0261

12711 Towne Park Way

Phone: 866-566-0261

1230 South Hurstbourne Parkway

Phone: 866-566-0261

7400 New Lagrange Rd

Phone: 866-566-0261

10110 Dixie Highway

Phone: 866-566-0261

539 South Fourth Street

Phone: 866-566-0261

200 Abraham Flexner Way

Phone: 866-566-0261

210 East Gray Street

Phone: 866-566-0261

4420 Dixie Highway

Phone: 866-566-0261

12701 Towne Park Way

Phone: 866-566-0261

5601 South 3rd Street

Phone: 866-566-0261

175 S English Station Rd

Phone: 866-566-0261

5601 S 3rd St Basement

Phone: 866-566-0261

3430 Newburg Rd Ste 110

Phone: 866-566-0261

3901 Dutchmans Ln Ste 106

Phone: 866-566-0261

1169 Eastern Pkwy Ste G10

Phone: 866-566-0261

4130 Dutchmans Ln Ste 102

Phone: 866-566-0261

9812 Vista Hills Boulevard

Phone: 866-566-0261

8005 Blue Lick Rd.

Phone: 866-566-0261

10284 Shelbyville Road

Phone: 866-566-0261

3430 Newburg Rd Ste 106

Phone: 866-566-0261

100 High Rise Drive

Phone: 866-566-0261

1169 Eastern Pkwy Ste 2343

Phone: 866-566-0261

444 South Main Street

Phone: 866-566-0261

344 East Center Street

Phone: 866-566-0261

210 Marie Langdon Dr

Phone: 866-566-0261

3060 Wolf Hollow Rd

Phone: 866-566-0261

107 S. 7th Street

Phone: 866-566-0261

1111 Medical Center Circle

Phone: 866-566-0261

927 Kenton Station Drive

Phone: 866-566-0261

927 Kenton Station Dr

Phone: 866-566-0261

1350 Medical Park Dr

Phone: 866-566-0261

1 East Mcdonald Parkway

Phone: 866-566-0261

9549 Ky Route 122

Phone: 866-566-0261

615 Upper Johns Creek Road

Phone: 866-566-0261

One South Creek Dr

Phone: 866-566-0261

284 Us Highway 60 W

Phone: 866-566-0261

250 Foxglove Drive, Ste 4

Phone: 866-566-0261

250 Foxglove Dr Ste 8

Phone: 866-566-0261

145 Newcomb Avenue

Phone: 866-566-0261

300 South 8th Street

Phone: 866-566-0261

803 Popular Street

Phone: 866-566-0261

1000 S. 12th Street

Phone: 866-566-0261

1104 Lowell Street

Phone: 866-566-0261

391 W. Tom T Hall Blvd

Phone: 866-566-0261

2211 Mayfair Dr. Ste 101

Phone: 866-566-0261

3245 Mount Moriah Avenue

Phone: 866-566-0261

922 Triplett St Ste 10

Phone: 866-566-0261

2831 New Hartford Road

Phone: 866-566-0261

2200 East Parrish Avenue

Phone: 866-566-0261

3600 Frederica Street

Phone: 866-566-0261

2200 E. Parrish Bldg. C 104

Phone: 866-566-0261

815 E. Parish Ave. Ste. 230

Phone: 866-566-0261

2605 Kentucky Ave

Phone: 866-566-0261

127 Aiben Barkley Drive

Phone: 866-566-0261

2301 Kentucky Ave

Phone: 866-566-0261

4630 Village Square Drive

Phone: 866-566-0261

4630 Village Sq Dr, Suite 103

Phone: 866-566-0261

225 Medical Center Dr Ste 408

Phone: 866-566-0261

1530 Lone Oak Rd

Phone: 866-566-0261

2535 Broadway Street

Phone: 866-566-0261

1700 Kentucky Avenue

Phone: 866-566-0261

2501 Kentucky Ave

Phone: 866-566-0261

625 James Trimble Blvd.

Phone: 866-566-0261

387 Town Mountain Rd

Phone: 866-566-0261

255 Church St Ste 104

Phone: 866-566-0261

387 Town Mountain Rd Ste 203

Phone: 866-566-0261

180 Town Mountain Road

Phone: 866-566-0261

911 S. Bypass Road

Phone: 866-566-0261

50 Weddington Branch Road

Phone: 866-566-0261

121 Virginia Avenue

Phone: 866-566-0261

1010 Medical Center Drive

Phone: 866-566-0261

400 University Drive

Phone: 866-566-0261

253 University Drive

Phone: 866-566-0261

1110 Lancaster Rd

Phone: 866-566-0261

646 University Shopping Ctr

Phone: 866-566-0261

1625 S. Nashville Road

Phone: 866-566-0261

113 1/2 E Public Square

Phone: 866-566-0261

101 Stonecrest Road

Phone: 866-566-0261

189 Adam Shepherd Pkwy

Phone: 866-566-0261

1707 Cedar Grove Road #9

Phone: 866-566-0261

401 Bogle St Ste 205

Phone: 866-566-0261

340 Bogle St Ste 103

Phone: 866-566-0261

68 E. Elkins Street

Phone: 866-566-0261

226 Medical Plaza Ln.

Phone: 866-566-0261

226 Medical Plaza Ln

Phone: 866-566-0261

998 Us Hwy. 25w South

Phone: 866-566-0261

1426 W Lexington Ave

Phone: 866-566-0261

225 Hospital Drive

Phone: 866-566-0261

1107 West Lexington Avenue

Phone: 866-566-0261

505 Shoppers Dr Ste 3

Phone: 866-566-0261

Instant Drug Testing

Standard laboratory drug tests provide negative results back in 1 – 2 business days. In special cases, specimens have to go on for further testing and results will take 2 – 3 business days or longer. Ask about availability for instant drug testing in selected facilities throughout Kentucky. All non-negative drug tests must go to a SAMHSA certified laboratory for confirmation testing.

Types of Testing Available

At US Drug Test Centers, we offer any and all types of drug and alcohol testing you may require, including but not limited to:

Drug-Free Workplace Programs in Kentucky

Marijuana is the third most popular recreational drug in Kentucky behind only alcohol and tobacco. Kentucky has a drug free workplace program - 803 KAR 25:280. The regulations for 803 KAR 25:280 were adopted on June 6, 2008. This regulation establishes the requirements for employers to apply and be certified by the Department of Workers' Claims for implementing a drug-free workplace program. Maintaining a drug-free workplace demonstrates an employer's willingness to promote social responsibility while providing a safe working environment for the employees and in exchange, the employer may be eligible and receive economic benefits such as a 5% discount on their workers' compensation insurance premium.

Requirements for a certified Kentucky Drug-Free Workplace Program:

  1. Written Policy
  2. Education and Training
  3. Employee Assistance Program (EAP)
  4. Alcohol and Drug Testing
  5. Confidentiality of Records
  6. Certification

Application Requirements Include:

  • Written Policy
  • Distribution of documents to employees
  • A statement identifying alcohol and drug tests
  • A statement posted at place of employment
  • A statement describing the Employer's Assistance Program (EAP
  • A description of the alcohol and substance abuse education and awareness training program
  • A statement describing the confidentiality

In addition, an eleven-panel drug test is required, which tests for the following substances:

  • Amphetamines
  • Cannabinoids/THC
  • Cocaine
  • Opiate
  • Phencyclidine (PCP)
  • Benzodiazepines
  • Propoxyphene
  • Methaqualone
  • Methadone
  • Barbiturates
  • Synthetic narcotics (expanded opiates)

Specific to the Mining industry is a requirement for a drug-free workplace written policy. In mining, 0.04 is alcohol positive. At least 5% of workers comp premium discount exists for mining operation with qualified drug-free workplace including an EAP. A company that is a licensee of the Office of Mine Safety and Licensing (OMSL) can submit a written copy of their drug-free workplace program to OMSL for certification. If the program is certified by OMSL, then the licensee must submit proof of certification to KEMI to receive a 5% credit on their workers' compensation insurance premium. The program must remain in place the entire policy term to earn the credit.

US Drug Test Centers works with attorneys in Kentucky to assist for drug testing for their clients. Attorneys often need to order drug tests for divorce cases, child custody cases and accident investigations.

If you need a drug test today in Kentucky, call today. Our network facilities including all LabCorp facilities and all Quest Diagnostics facilities are available for immediate drug testing with one phone call to schedule. Drug and alcohol tests are available for individuals and for employers in all areas of Kentucky.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.