South Dakota Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


Methamphetamine and heroin have no boundaries when it comes to South Dakota metropolitan and rural communities. With a rise in overdoses since 2015, these two culprits continue to be the most common drugs used in the state. In the past 5 years hospitals have reported an increase in opioid related cases and on average most South Dakota residents repot a higher level of alcohol intake than the national average.

US Drug Test Centers have 84 locations in South Dakota and aim to be here for residents and visitors in need of information about drug and alcohol testing. With the statistics rising, it is vital that the community knows where to obtain drug and alcohol tests that can be done quickly and accurately. Most of our locations are open daily. You can also call to speak to a representative about your drug and alcohol testing needs.

We offer ETG and DOT testing as well as 5 and 10 panel screenings. If you need a test for work or personal reasons, we have your drug and alcohol testing need taken care of. Find more information about online drug tests on our website.

Finding a Drug Test Center Near You

US Drug Tests Centers can be found in all major cities in South Dakota, with 12 locations in Sioux Falls including one adjacent to the Avera McKennan Hospital and University in zip codes 57105, 57104, 57106, 57108 and 57110. We also have locations in Aberdeen (4), Brookings (3 including one less than 4 minutes form the Brookings Regional hospital), Watertown (4). If you are planning a trip to Mount Rushmore and need a test during your travels we have locations in Custer 19.7 miles south and 11 in Rapid City, Pennington County, zip codes 57701 and 57702.

See below for a complete list of our locations in South Dakota.

US Drug Test Centers

US Drug Test Centers is the complete, drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty.We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing.

We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. At USDTC, we can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings.

Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program.

We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

83 Testing locations in South Dakota

13 135th Street Southwest

Phone: 866-566-0261

209 Ramsland Street

Phone: 866-566-0261

405 Whitecar Avenue

Phone: 866-566-0261

102 Major Allen St

Phone: 866-566-0261

803 E. Milbank Avenue

Phone: 866-566-0261

1401 10th Avenue West

Phone: 866-566-0261

1730 North Haines Avenue

Phone: 866-566-0261

2201 Jackson Blvd Ste 102

Phone: 866-566-0261

2820 Mount Rushmore Rd

Phone: 866-566-0261

2006 Mount Rushmore Road

Phone: 866-566-0261

2116 Jackson Boulevard

Phone: 866-566-0261

1303 North Lacrosse Street

Phone: 866-566-0261

1010 West 1st Street

Phone: 866-566-0261

116 West Main Street

Phone: 866-566-0261

2100 South Marion Road

Phone: 866-566-0261

2221 West Russell Street

Phone: 866-566-0261

900 East 54th St. North, Suite

Phone: 866-566-0261

600 South Cliff Avenue

Phone: 866-566-0261

5027 South Bur Oak Place

Phone: 866-566-0261

138 East Hudson Street

Phone: 866-566-0261

1445 North Ave Ste 2

Phone: 866-566-0261

1440 North Main Street

Phone: 866-566-0261

931 2nd Avenue Southwest

Phone: 866-566-0261

901 4th Street Northwest

Phone: 866-566-0261

1401 W. First Street

Phone: 866-566-0261

745 East 8th Street

Phone: 866-566-0261

Drug Free Workplace Program

For employers US Drug Test Centers can help provide a comprehensive drug free workplace program including employee drug testing. Both DOT and non-DOT programs are available for complete compliance with Federal, State and local regulations. US Drug Testing Center works with businesses of all types, with an emphasis on industries where the substance abuse challenges are the greatest.

Comprehensive drug free workplace programs include:

  • A Written Substance Abuse Policy
  • Employee Awareness and Education
  • Supervisor Training
  • An Employee Assistance Plan of Action
  • Drug and Alcohol Testing

US Drug Test Centers provides effective drug-free program consisting of the five elements listed above that, together, provide a full, comprehensive management and training program designed specifically to meet the needs of your company. US Drug Test Centers can help your company get started with a drug-free program that's right for you, or help you put in place the right processes for maintaining your program, depending upon your specific situation.

South Dakota Laws on Drug Testing

South Dakota has no law restricting or prohibiting drug testing by private employers. South Dakota law does not address drug testing in private employment. This means that private employers are not prohibited or restricted from drug testing applicants and employees.

Immediate Testing

All US Drug Testing Center locations are able to provide immediate drug and alcohol testing for individuals and employers, when the situation requires immediate testing with expedited results. Immediate testing means that the testing itself is done same-day, or immediately, with the results usually reported back the next business day. This immediate drug and alcohol testing is available for situations such as:

Many attorneys in South Dakota work with our US Drug Test Centers to order probation & court ordered drug and or alcohol tests for their clients.

For individuals, personal drug and alcohol testing is always available with same day service. Our testing programs include:

Do you need a drug test today in the State of South Dakota? US Drug Test Centers is a nationwide company with locations throughout SOUTH DAKOTA for drug and alcohol testing. Drug and alcohol testing with immediate service is available for individuals for personal drug tests and for employers for employee drug testing.

Call now 1-866-566-0261 for service at any of our many facilities in the State of South Dakota.

In South Dakota, US Drug Test Centers has facilities throughout the State in all cities, with multiple facilities in Sioux Falls, Rapid City, Aberdeen, Brookings, Watertown, Yankton, Pierre, Huron and Vermillion.

About South Dakota

From the Badlands to Mount Rushmore, South Dakota has no shortage of places to visit. For visitors who enjoy fishing, exploring caves or visiting historical landmarks, South Dakota does not disappoint. With a population of South Dakota under 900,000 and the majority of its residents living in Buffalo County, South Dakota, like other Midwest states, caters to a large rural population of towns with less than 3,000 residents.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.