Knoxville, Tennessee Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


If you're looking for drug and alcohol screening in the Knoxville, Tennesse area, US Drug Test Centers can help. Here are just a few of the ways we can assist you.

Drug and Alcohol Testing in Knoxville, Tennessee

Employee and Pre-Employment Drug Testing

A drug-free facility benefits everyone in the workplace. Committing to a drug-free policy can also reduce your exposure to liability related to drug or alcohol incidents. US Drug Test Centers can help your company implement and maintain a drug-free environment with our complete range of drug testing services.

We offer fast results for DOT and non-DOT drug tests - 93% of our negative drug test results are reported in 24 to 48 hours. Instant testing for non-DOT programs is also available with same-day negative results.

Online Training for Businesses in Knoxville, Tennessee

US Drug Test Centers offer a variety of affordable online training programs for your supervisors. There are DOT and non-DOT courses. Your supervisors may have already taken online drug and alcohol training. These courses are revised and updated annually as new information becomes available. It's recommended that supervisor refresher training is completed every 12 to 18 months.

Legally Mandated Drug Tests

We can perform drug tests required by probation or court orders. This service can be provided on a daily basis or as necessary. Our drug testing specialists are committed to the highest standards. This ensures properly administered tests and accurate results.

Personal Drug Testing

Our drug testing facilities in Knoxville can perform immediate drug tests for individuals which may be required in the following situations:

  • Court order
  • Probation
  • Divorce hearings
  • Custody hearings

Knoxville's Most Accurate Drug and Alcohol Testing

How do you know if a test is accurate? All of US Drug Test Centers' specimens go to a SAMHSA-certified laboratory for testing. SAMHSA has an active advisory board that keeps its employees and administrators abreast of current and changing laws when it comes to drug testing. There are Mandatory Guidelines for Federal Employee Drug Testing Programs that must carry consistencies throughout every agency and company that SAMHSA laboratories supplies. The advisory board has professionals that are uniquely qualified in their respective fields to review scientific evidence in the area of drug and alcohol treatment and testing practices.

SAMHSA Report for Tennessee

Reports published by the Substance Abuse and Mental Health Services Administration estimate that there are 378,000 people over the age of 12 who are dependent or abuse illicit drugs or alcohol in Tennessee. Though Knoxville is nowhere near epidemic levels, drug testing and drug-free workplace programs help to directly address substance abuse issues in a community.

About Knoxville, Tennessee

Knoxville is the county seat of Knox County, Tennessee. It has a population over 180,000. The Knoxville Metro Area is located in eastern Tennessee and consists of Anderson, Blount, Knox, Loudon, and Union counties. Other principal cities in the area are Morristown and Sevierville.

Retail, finance, tech, and manufacturing all play vital roles in Knoxville's economy. Some major corporations in the area are Tennessee Valley Authority, Regal Entertainment Group, and Pilot Flying J.

Knoxville is home to Knoxville Botanical Gardens and Arboretum, Knoxville Museum of Art, and the Knoxville Zoo. Shakespeare on the Square, Symphony in the Park, and the International Biscuit Festival are just a few of the many events and festivals that take place in Knoxville all year long.

Have more questions? Visit our FAQs page for answers!

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

32 Testing locations in Knoxville, Tennessee

7349 Chapman Hwy

Knoxville, Tennessee 37920

Phone: 866-566-0261

9203 Kingston Pike

Knoxville, Tennessee 37922

Phone: 866-566-0261

101 Glenleigh Ct

Knoxville, Tennessee 37934

Phone: 866-566-0261

5917 Rutledge Blvd

Knoxville, Tennessee 37924

Phone: 866-566-0261

2725 Asbury Rd Ste 103

Knoxville, Tennessee 37914

Phone: 866-566-0261

4711 Centerline Drive Suite 100

Knoxville, Tennessee 37917

Phone: 866-566-0261

4711 Centerline Dr

Knoxville, Tennessee 37917

Phone: 866-566-0261

9815 Cogdill Rd

Knoxville, Tennessee 37932

Phone: 866-566-0261

116 Glenleigh Court

Knoxville, Tennessee 37934

Phone: 866-566-0261

120 Suburban Rd

Knoxville, Tennessee 37923

Phone: 866-566-0261

1300 Old Weisgarber Rd

Knoxville, Tennessee 37909

Phone: 866-566-0261

11201 West Point Drive

Knoxville, Tennessee 37934

Phone: 866-566-0261

901 Merchant Drive

Knoxville, Tennessee 37912

Phone: 866-566-0261

108b W Inskip Dr # B

Knoxville, Tennessee 37912

Phone: 866-566-0261

108 W Inskip Dr Ste B

Knoxville, Tennessee 37912

Phone: 866-566-0261

7000 Maynardville Hwy

Knoxville, Tennessee 37918

Phone: 866-566-0261

4306 Asheville Hwy

Knoxville, Tennessee 37914

Phone: 866-566-0261

1826 Ailor Ave

Knoxville, Tennessee 37921

Phone: 866-566-0261

1932 Alcoa Hwy, Bldg C,#155

Knoxville, Tennessee 37920

Phone: 866-566-0261

4206 Sutherland Avenue

Knoxville, Tennessee 37919

Phone: 866-566-0261

4605 Papermill Drive

Knoxville, Tennessee 37909

Phone: 866-566-0261

4605 Papermill Dr

Knoxville, Tennessee 37909

Phone: 866-566-0261

8712 Asheville Hwy

Knoxville, Tennessee 37924

Phone: 866-566-0261

9135 Middlebrook Pike

Knoxville, Tennessee 37923

Phone: 866-566-0261

2725 Gov John Sevier Hwy. Se

Knoxville, Tennessee 37914

Phone: 866-566-0261

2725 Gov. John Sevier Highway,

Knoxville, Tennessee 37914

Phone: 866-566-0261

8350 Kingston Pike

Knoxville, Tennessee 37919

Phone: 866-566-0261

7200 Strawberry Plains Pike

Knoxville, Tennessee 37914

Phone: 866-566-0261

4028 Papermill Drive Northwest

Knoxville, Tennessee 37909

Phone: 866-566-0261

1320 Papermill Pointe Way

Knoxville, Tennessee 37909

Phone: 866-566-0261

1 Veterans Way

Knoxville, Tennessee 37931

Phone: 866-566-0261

10820 Parkside Drive

Knoxville, Tennessee 37934

Phone: 866-566-0261