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Tennessee Drug Testing | Drug & Alcohol Testing Tennessee

Tennessee Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


Tennessee is home to over 6 million residents. Known for the Great Smokey Mountains and music legends like Elvis Presley, Muddy Waters, Johnny Cash and Dolly Parton. There is no shortage of culture and beauty from Memphis to Johnson City; a US Drug Test Center is guaranteed to be near by to assist you with your drug and alcohol testing needs.

80% of crimes in Tennessee have been linked to illegal drugs. With the state working hard to combat heroin, the citizens who struggle with addiction have been forced to move towards prescription opioids. Tennessee ranks 14th in the nation for drug abuse and 3rd in opioid prescriptions written per capita. Each year in Tennessee there are over 100,000 people abusing other illegal drugs such as cocaine and crack cocaine.

With statistics like this it is important for the community to know where to seek help. US Drug Test Centers are here to help guide you with your drug and alcohol testing needs. We offer more than 450 locations throughout Tennessee where you can obtain, take and get information regarding drug and alcohol tests. Including; ETG, DOT hair follicle, breath alcohol tests and in many cases same day testing results are possible. Call us today to speak to a representative about your options or where to take a test.

You can find a US Drug Test Center in Shelby County; 4 in Bartlett, over 40 in Memphis alone including zip codes 3810, 38111, and 38113 near Downtown and the Medical District. In Davidson County with 28 locations in Nashville. If you’re passing threw to visit the Grand Ole Opry, we have a location less than 7 miles away in zip code 37214, or the Country Music Hall of Fame we have a location less than 2 miles away in zip code 37203. In Knox County we have over 30 locations in Knoxville. Outside of the largest cities we also have locations in Somerville, Waverly, Fairview and Middleton and many more.

Drug and alcohol testing in Tennessee is available for:
DOT Drug Testing • DOT Consortium Random Programs • Breath Alcohol Testing (BAT) • Drug Free Workplace Programs • Court Ordered Drug Testing • EtG Alcohol Testing • Hair Follicle Drug Testing • Probation Drug Testing • Legal Drug Testing • Employment Drug Testing • Personal Drug Testing • Child Custody and Divorce Cases
Individuals and employers can call US Drug Test Centers at 866-566-0161 today for drug and alcohol testing. Our drug testing centers throughout Tennessee require pre-registration for all drug and alcohol testing, so call US Drug Test Centers today for service. Same day service and same day results can be available for most urine drug testing.

457 Testing locations in Tennessee

217 Airport Plaza Blvd Ste B

Phone: 866-566-0261

409 Congress Parkway North

Phone: 866-566-0261

2324 Congress Parkway South

Phone: 866-566-0261

7424 Us Highway 64 Ste 111

Phone: 866-566-0261

7777 North Brother Boulevard

Phone: 866-566-0261

330 Franklin Rd # 135a-595

Phone: 866-566-0261

330 Franklin Rd # 135a-323

Phone: 866-566-0261

330 Franklin Rd # 135a-321

Phone: 866-566-0261

791 Old Hickory Boulevard

Phone: 866-566-0261

350 Blountville Hwy Ste 101

Phone: 866-566-0261

6101 Enterprise Park Drive

Phone: 866-566-0261

6016 Shallowford Rd Ste 200

Phone: 866-566-0261

931 Spring Creek Road Ste 200

Phone: 866-566-0261

600 N Holtzclaw Ave Ste 210

Phone: 866-566-0261

2831 Wilma Rudolph Boulevard

Phone: 866-566-0261

3535 Keith Street Northwest

Phone: 866-566-0261

1060 Peerless Crossing Ste 200

Phone: 866-566-0261

2336 Keith Street Northwest

Phone: 866-566-0261

2650 Executive Park Northwest,

Phone: 866-566-0261

2305 Chambliss Avenue Northwest

Phone: 866-566-0261

1107 Charles G. Seivers Blvd.

Phone: 866-566-0261

315 N Washington Ave # 165

Phone: 866-566-0261

275 S Walnut Bend Rd Ste 101

Phone: 866-566-0261

60 Crossville Medical Grp Dr

Phone: 866-566-0261

60 Crossville Medical Group Dr

Phone: 866-566-0261

4589 Rhea County Highway

Phone: 866-566-0261

455 Chickamauga Dr. Ste 104

Phone: 866-566-0261

9400 Rhea County Highway

Phone: 866-566-0261

1355 Flowering Dogwood Ln Ste

Phone: 866-566-0261

1355 Flowering Dogwood Lane

Phone: 866-566-0261

225 Noah Drive Suite 360

Phone: 866-566-0261

1340 N. Grundy Quarles Highway

Phone: 866-566-0261

300 Steam Plant Rd Ste 300

Phone: 866-566-0261

208 Suncrest St Suite 2

Phone: 866-566-0261

2055 East Andrew Johnson Hwy Suite 2a

Phone: 866-566-0261

2497 S. Roane St Suite 110

Phone: 866-566-0261

2075 Pleasant Plains Exd

Phone: 866-566-0261

1950 Industrial Boulevard

Phone: 866-566-0261

200 Medtech Parkway Ste. 108

Phone: 866-566-0261

401 Princeton Rd. Occup. Hlth

Phone: 866-566-0261

4711 Centerline Drive Suite 100

Phone: 866-566-0261

1932 Alcoa Hwy, Bldg C,#155

Phone: 866-566-0261

2725 Gov John Sevier Hwy. Se

Phone: 866-566-0261

2725 Gov. John Sevier Highway,

Phone: 866-566-0261

7200 Strawberry Plains Pike

Phone: 866-566-0261

4028 Papermill Drive Northwest

Phone: 866-566-0261

1320 Papermill Pointe Way

Phone: 866-566-0261

919 E. Central Ave Ste 101 B

Phone: 866-566-0261

100 Physicians Way, Ste. 110

Phone: 866-566-0261

689 Medical Park Dr Ste 103

Phone: 866-566-0261

460 Medical Park Dr Ste 103

Phone: 866-566-0261

1080 N. Ellington Parkway

Phone: 866-566-0261

701 Morganton Square Drive

Phone: 866-566-0261

1810 Robert C Jackson Drive

Phone: 866-566-0261

3965 South Mendenhall Road

Phone: 866-566-0261

3965 S Mendenhall Rd Ste 6

Phone: 866-566-0261

3233 Players Club Parkway

Phone: 866-566-0261

2725 Kirby Road Suite 15

Phone: 866-566-0261

5308 Cottonwood Rd Ste 1-a

Phone: 866-566-0261

6100 Primacy Pkwy Ste 106

Phone: 866-566-0261

6400 Shelby View Dr Ste 101

Phone: 866-566-0261

8485 Us Highway 64 Ste 101

Phone: 866-566-0261

850 West Third Street, Suite A

Phone: 866-566-0261

1633 West Morris Boulevard

Phone: 866-566-0261

1340 Northwest Broad Street

Phone: 866-566-0261

519 Donelson Pike Ste 107

Phone: 866-566-0261

1400 Donelson Pike Ste B10

Phone: 866-566-0261

501 Metroplex Drive Ste 105

Phone: 866-566-0261

1916 Patterson St Ste 103

Phone: 866-566-0261

441 South Illinois Avenue

Phone: 866-566-0261

200 Stonecrest Boulevard

Phone: 866-566-0261

1171 Highway 11 E, Ste. 101

Phone: 866-566-0261

701 E Reelfoot Ave Ste 600

Phone: 866-566-0261

1211 Dinah Shore Boulevard

Phone: 866-566-0261


Today, millions of Americans struggle with substance abuse-related issues, both at home as well as in the workplace. Companies in Tennessee value their employees and customers and recognize the adverse effects that substance abuse—including the use of illegal drugs, the abuse of alcohol, and misuse of prescription drugs and over-the-counter medications—can have on the work environment. Employees who use drugs and abuse alcohol are less productive, less reliable, and pose a direct threat to the safety and well-being of others. These companies in Tennessee implement drug free workplace programs. These same Tennessee companies receive a 5% premium credit on their workers’ insurance policy.

US Drug Test Centers can assist employers in implementing a compliant Tennessee Drug Free Workplace program or a DOT drug and alcohol testing program including the random consortium program.

In Tennessee, as defined in the Drug Free Workplace Rules and Guidelines, "Medical Review Officer" or "MRO" means a licensed physician, employed with or contracted with a covered employer, who has knowledge of substance abuse disorders, laboratory testing procedures and chain of custody collection procedures; who verifies positive, confirmed test results; and who has the necessary medical training to interpret and evaluate an employee's positive test result in relation to the employee's medical history or any other relevant biomedical information. The MRO should be used to review all positive tests with the employee or applicant before the employer is advised of the test results. Using an MRO provides the employer with a medically qualified interpretation of a positive test result, which would be defensible in a court of law. The MRO helps to protect both the employer and the employee.

A misconception is that drug testing is prone to inaccuracy with so-called "false" positives. Several years ago, some over-the-counter drugs such as ibuprofen or diet pills could cause false positives for illicit drugs. Today, tests have been refined to the point where this does not occur. Courts favor employee testing that is based on procedures that are clear, fair, consistent, and communicated in a written policy statement.

Tennessee is a landlocked state in the U.S. South. Its capital, centrally located Nashville, is the heart of the country-music scene, with the long-running “Grand Ole Opry,” the Country Music Hall of Fame and Museum and a legendary stretch of honky-tonks and dance halls. Memphis, in the far southwest, is the home of Elvis Presley’s Graceland and the blues clubs of Beale Street. Tennessee borders eight other states: Kentucky and Virginia to the north; North Carolina to the east; Georgia, Alabama, and Mississippi on the south; Arkansas and Missouri on the Mississippi River to the west. Tennessee ties Missouri as the state bordering the most other states. College football fans love their Tennessee Volunteers football team.

For drug and alcohol testing services in Tennessee, same day service is just a phone call away. Employers and individuals can call today to pre-register drug tests in all areas of Tennessee. Drug testing programs are available for small and large businesses. Call US Drug Test Centers today at 866-566-0261!

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.