Chicago, Illinois Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


111 Testing locations in Chicago, Illinois

1460 N Halsted St Ste 201

Chicago Illinois 60642

Phone: 866-566-0261

8751 S Greenwood Ave Ste 106

Chicago Illinois 60619

Phone: 866-566-0261

3000 N. Halsted

Chicago Illinois 60657

Phone: 866-566-0261

4126 N Milwaukee Ave

Chicago Illinois 60641

Phone: 866-566-0261

4801 W Peterson Ave Ste 205

Chicago Illinois 60646

Phone: 866-566-0261

2800 N Sheridan Rd Ste G1

Chicago Illinois 60657

Phone: 866-566-0261

1431 N Western Ave Ste 137

Chicago Illinois 60622

Phone: 866-566-0261

614 W Monroe St

Chicago Illinois 60661

Phone: 866-566-0261

4025 N Western Ave

Chicago Illinois 60618

Phone: 866-566-0261

25466 Network Pl

Chicago Illinois 60673

Phone: 866-566-0261

7107 West Belmont Avenue

Chicago Illinois 60634

Phone: 866-566-0261

4753 N Elston Ave

Chicago Illinois 60630

Phone: 866-566-0261

36 West Randolph Street

Chicago Illinois 60601

Phone: 866-566-0261

5444 South Archer Avenue

Chicago Illinois 60638

Phone: 866-566-0261

3520 S Ashland Ave

Chicago Illinois 60609

Phone: 866-566-0261

3520 S. Ashland Ave

Chicago Illinois 60609

Phone: 866-566-0261

3198 Solutions Center

Chicago Illinois 60677

Phone: 866-566-0261

211 E. Ohio Ste 2019

Chicago Illinois 60611

Phone: 866-566-0261

6224 S Pulaski Rd

Chicago Illinois 60629

Phone: 866-566-0261

900 East 103rd Street

Chicago Illinois 60628

Phone: 866-566-0261

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Phone: 866-566-0261

US Drug Test Centers has the most drug testing locations in the Chicagoland area. We offer a variety of drug tests that are fast, affordable, accurate, and convenient. Not only can we perform drug testing in the Chicagoland area, but also nationwide. Give our office a call to get scheduled today!

Nationwide Immediate Service

Both employers and individuals often find situations where they need to order a drug test for immediate service or on going service for themselves or their employees. US Drug Test Centers is a nationwide company with over 20,000 locations ready to serve you, no matter where you are! Just one phone call to 866-566-0261 can arrange immediate drug or alcohol testing. Call today for service and get your drug testing completed. Our drug and alcohol testing services are provided for individuals needing drug testing for probation, court ordered testing, self-testing, drug testing of teenagers and more. For employers and staffing agencies, our drug testing centers can arrange for an immediate drug test for your applicant or employee anywhere in the country. Call 866-566-0261 for immediate service for drug testing Chicago IL.

Drug and Alcohol Testing Is Serious Business For Your Business

The federal government estimates that drug and alcohol abuse in the workplace and its related events, costs American businesses up to $80 billion a year, and that is just in lost productivity alone. According to government statistics, employees who use drugs are three times more likely to be late to work, over three-and-a-half times more likely to be involved in a workplace accident, and five times more likely to file workers' compensation claims.

US Drug Test Centers is a nationwide network of testing centers whose mission is to provide your business with immediate testing solutions for your Drug and alcohol testing and drug-free workplace needs. We provide complete DOT drug testing and compliance programs, and drug-free workplace program needs, as well as being able to establish an ongoing Chicago drug testing and drug-free workplace program for your business.

DOT Compliant Drug and Alcohol Testing Programs

If you are a DOT-regulated company and need to have a DOT compliant drug and alcohol program in place, US Drug Test Centers are the professionals in establishing and servicing DOT compliant testing programs for the transportation industry. We strictly adhere to DOT compliance standards in all of our testing, which means when you use US Drug Test Centers, you can be rest assured that your program will pass a DOT audit anytime. Our drug screening Chicago is always up to date.

We Provide DOT-Compliant Testing Services to the Following Industries

US Drug Test Centers are here to provide DOT-compliant drug and alcohol testing and drug-free workplace programs to DOT regulated industries such as:

    • Trucking Industry
    • Aviation Industry
    • Railroad Industry
    • Public Transportation
    • Pipeline Industry
    • Maritime Industry

Call today to order your drug test Chicago.

About Chicago, Illinois

The City of Chicago is the county seat of Cook County, Illinois. It is the third largest city in the United States with a population of over 2,700,000 people. The Chicago Metro Area consists of Cook, DuPage, Kane, Lake, and Will counties in Illinois and Lake County, Indiana. Aurora, Elgin, Joliet, Kenosha, and Naperville are other large cities in the area.

The city of Chicago is home to 12 Fortune Global 500 companies and 17 Financial Times 500 companies. It is one of the major financial centers of the world. Manufacturing, printing, publishing, and food processing are other important industries in the city.

There are many parks, museums, and attractions in Chicago. Lincoln Park has a zoo and a conservatory. The River North Gallery District features one the nation's largest concentration of contemporary art galleries. Museum of Science and Industry is located in Hyde Park.

Facts about Chicago

  • Chicago was where the first fission reaction was created by a group of scientists working with physicist Enrico Fermi, under the football stands of the University of Chicago's stadium.
  • Chicago is located almost equally distant from the Pacific and Atlantic oceans.
  • Chicago has the largest collection of Impressionist paintings in the world, outside of the Louvre museum in Paris.
  • Chicago is home to the world's first skyscraper, the Home Insurance Company, which was built in 1885.

SAMHSA Report for Illinois

Reports published by the Substance Abuse and Mental Health Services Administration estimate that there are 820,000 people over the age of 12 who are dependent or abuse illicit drugs or alcohol in Illinois. Though Chicago is nowhere near epidemic levels, drug testing and drug free workplace programs help to directly address substance abuse issues in a community.

Immediate Service Available for DOT Random Testing Programs

Same day service is available to enroll in our DOT random consortium for owner operators and small trucking companies in Chicago. Certificates of compliance are issued same day when drivers are enrolled into our consortium pool or a stand-alone pool. Programs can be set up immediately. Contact US Drug Test Centers to quickly set up a consortium pool or a stand-alone pool. US Drug Test Centers has a special program for owner operators to participate in our random testing consortium. Same day service is available to enroll in our DOT Random Consortium.

DOT Drug and Alcohol Testing Program Compliance in Chicago

There are many specific components a company must have in place in order to be compliant with DOT regulations. US Drug Test Centers can guarantee your company will be in compliance by assisting in the creation of the follow documents, policies, and procedures:

  • Designated Employer Representative (DER)
  • Written Policy
  • Regulations on file
  • Pre-employment Testing
  • Random, Post-Accident, Reasonable Suspicion, Return to Duty and Follow-up Testing
  • Employee Education
  • Supervisor Training
  • Previous Employer Checks
  • Removal of employees from safety sensitive positions when positive or refusal to test
  • Substance Abuse Professional


Employees are Responsible for Their Safety and the Safety of Others

Employees must take reasonable care for their own health and safety and that of others. They must cooperate with the employer when actions are taken to comply with company alcohol and drug policy regulations. It is considered a good practice for employers to remove employees from their act of duty immediate when they have failed the drug test or reported for services under the influence of alcohol or illicit drugs.

Healthcare Professional Drug and Alcohol Testing in Chicago, Illinois

Our Healthcare Professional Panel is also available in Chicago. This panel tests for specific, highly addictive prescription medications that are readily accessible to workers in this field. This is a urine test which determines the presence or absence of the most abused prescription drugs or their metabolites. Our Healthcare Professional Panel is also an excellent standard for allied health students.

Chicago's Most Accurate Drug and Alcohol Testing

How do I know if the test is accurate? All of US Drug Test Centers' specimens go to a SAMHSA certified laboratory for testing. SAMHSA has an active advisory board that keeps its employees and administrators abreast of current and changing laws when it comes to drug testing. There are Mandatory Guidelines for Federal Employee Drug Testing Programs that must carry consistencies throughout every agency and company that SAMHSA laboratories supplies. The advisory board has professionals that are uniquely qualified in their respective fields to review scientific evidence in the area of drug and alcohol treatment and testing practices.

Have more questions, visit our FAQs page for answers!

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.


Frequently Asked Questions About Illinois Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated Dec 2022

Illinois Drug Testing FAQs

Do employers in Illinois need to comply with mandatory state-specific rules when drug & alcohol testing?
No. Illinois does not have any mandatory drug testing statutes or regulations but it is recommended.
Is a Drug-Free Workplace policy required in Illinois?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with the Illinois’ voluntary law).
Are employers in Illinois able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may leverage an ‘Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Does Illinois authorize marijuana (THC) or cannabidiol (CBD) use?
Yes. This state authorizes personal adult use (effective 1/1/2020) and the medical use of marijuana. The medical marijuana law/pilot program impacts the workplace. Employers are prohibited from discriminating against an individual’s status authorize medical marijuana patient. Employers are permitted to have a zero-tolerance policy and discipline due to a positive drug test result. An employer may consider an employee to be impaired or under the influence of cannabis if the employer has a good faith belief that an employee manifests specific articulable symptom(s) while at work. Some confusion has arisen from the states “Right to Privacy in the Workplace Act” which protects those who use “lawful products” from discrimination at work (marijuana will now be considered a lawful product in the state of IL).  However, Section 5(b) states: “This Section does not apply to the use of those lawful products which impairs an employee's ability to perform the employee's assigned duties.” 

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