Colorado Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


Colorado Drug And Alcohol Testing

The state of Colorado, like many in the United States, is struggling with the influx of illicit substances and the impact of drug and alcohol use. In 2015, Colorado was ranked the highest in the country for use of opioid painkillers, alcohol, cocaine and marijuana.

Additionally, in 2015 there were more deaths in Colorado as a result of drug and alcohol abuse than by car crashes. The rate of fatalities from opioid overdoses increased by more than a multiple of three between 2000 and 2015.

Drug Testing Facilities

US Drug Test Centers is proud to offer 370 immediate drug and alcohol testing locations in the state of Colorado. Some of our collection sites are available for same-day drug and alcohol screenings and walk-ins, including Alamosa, Aurora, and several other major cities in the state of Colorado. To schedule, simply call 1-866-566-0261 before 10 am on the morning of your test. Walk-in rapid drug and alcohol screening test collection services are also available at some of our testing centers.

For full-service drug testing in Colorado, contact US Drug Test Centers today. We offer pre-employment testing, DOT drug and alcohol testing, Non-DOT testing, random drug testing, reasonable suspicion and post-accident testing. Additionally, US Drug Test Centers also offers instant drug testing with same-day results.

See below for a full list of testing centers in Colorado.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

US Drug Test Centers

US Drug Test Centers

We are your complete drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty. We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing. We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. We can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings. Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program. We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.


Frequently Asked Questions About Colorado Drug Testing

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated Nov 2022

Colorado Drug Testing FAQs

Do employers in Colorado need to comply with mandatory state-specific rules when drug & alcohol testing?
No.  Colorado does not have any mandatory drug testing statutes or regulations. However, employers in the city of Boulder, CO face stringent drug testing rules, due to a city ordinance.
Is a Drug-Free Workplace policy required in Colorado?
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with Colorado’s voluntary law).
Are employers in Colorado able to use drug screening for any other claim defenses or state-specific financial benefits?
Yes. Employers may leverage a ‘Rebuttable Presumption of Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result to qualify for a 50% in benefit award. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Does Colorado authorize marijuana (THC) or cannabidiol (CBD) use?
Yes. Colorado permits the personal adult use and the medical use of marijuana for patients that meet specific medical conditions. The laws do not specifically impact employment drug testing programs.

369 Testing locations in Colorado

6609 County Road 114 South

Alamosa Colorado 81101

Phone: 866-566-0261

2115 Stewart St

Alamosa Colorado 81101

Phone: 866-566-0261

5293 Ward Rd

Arvada Colorado 80002

Phone: 866-566-0261

5511 West 56th Ave

Arvada Colorado 80002

Phone: 866-566-0261

6140 S Gun Club Rd

Aurora Colorado 80016

Phone: 866-566-0261

6140 K6 South Gun Club Road #2

Aurora Colorado 80016

Phone: 866-566-0261

15235 East 38th Avenue

Aurora Colorado 80011

Phone: 866-566-0261

10355 E. Iliff Avenue

Aurora Colorado 80247

Phone: 866-566-0261

3449 N Chambers Rd

Aurora Colorado 80011

Phone: 866-566-0261

3350 Peoria St

Aurora Colorado 80010

Phone: 866-566-0261

3797 Windsor Drive

Aurora Colorado 80011

Phone: 866-566-0261

14097 East Exposition Ave.

Aurora Colorado 80012

Phone: 866-566-0261

1444 South Potomac Street

Aurora Colorado 80012

Phone: 866-566-0261

1550 South Potomac Street

Aurora Colorado 80012

Phone: 866-566-0261

1550 S Potomac, Ste 325

Aurora Colorado 80012

Phone: 866-566-0261

16455 East 40th Circle

Aurora Colorado 80011

Phone: 866-566-0261

1250 South Buckley Road

Aurora Colorado 80017

Phone: 866-566-0261

1411 S Potomac St

Aurora Colorado 80012

Phone: 866-566-0261

14100 E Jewell Ave

Aurora Colorado 80012

Phone: 866-566-0261

14100 E. Jewell Ave

Aurora Colorado 80012

Phone: 866-566-0261

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Phone: 866-566-0261

Marijuana Use in a Legalized State

Countless employers in Colorado are implementing drug testing programs, during the hiring process, to filter our potential employees who smoke marijuana. Although medical marijuana and recreational marijuana are legal in Colorado, employers have every right to implement and maintain a drug-free workplace program, which includes testing for marijuana (medical or otherwise). Recent studies show an increase in the number of employers who have established drug testing programs in the workplace since recreational marijuana use was legalized in 2014.

The Colorado Supreme Court has issued a ruling on the topic of medical marijuana and employment practices. In Coats v. Dish Network, an employee sued for wrongful termination after he tested positive for marijuana. The plaintiff is a quadriplegic who used medical marijuana outside of working hours. Mr. Coats was a registered medical marijuana user, accessing the product in a manner consistent with state constitutional guarantees and state statute. The use of marijuana is still illegal under Federal law. Dish Network has a comprehensive drug free workplace policy. The court held in a unanimous opinion that the term "lawful activity" extends to both state and federal law.

According to Federal law, marijuana is a schedule I drug. Under that classification, marijuana "has a high potential for abuse…has no currently accepted medical use in treatment in the United States [and] there is a lack of accepted safety for use of the drug or other substance under medical supervision" [21 U.S.C 812(b)(1)].

Employers in the state of Colorado have a right to implement a employee drug testing program. The state of Colorado imposes no restrictions except in the City of Boulder. US Drug Test Centers offers both DOT and non-DOT drug testing programs for employers in Colorado. Setting up a workplace program is simple and affordable with your testing specialists from US Drug Test Centers.

Why Choose US Drug Test Centers

There are various reasons why you may have a need for a drug or alcohol test. US Drug Test Centers offers drug testing for individuals with same day service. Drug testing is available for personal reasons, probation drug testing, DUI drug testing, child custody cases, divorce cases and court-ordered programs. US Drug Test Centers also works with Colorado-based attorneys in setting up drug or alcohol testing for their clients. In many cases, same day drug test results are available on request.

US Drug Test Centers drug testing collection sites are available in all areas of Colorado including the City of Colorado Spring, Denver, City of Aurora, City of Fort Collins, City of Grand Junction, City of Lakewood, City of Thornton, City of Pueblo, City of Arvada, City of Westminster, City of Centennial, City of Boulder, City of Greeley, Town of Castle Rock, City of Longmont, City of Loveland, City and County of Broomfield,, City of Commerce City, City of Englewood, Town of Parker City of Littleton, City of Northglenn, City of Brighton, City of Wheat Ridge, City of Fountain and the City of Lafayette.

Call US Drug Test Centers at 866-566-0261 today for drug and alcohol, hair testing and EtG alcohol testing. Testing options and additional fees may vary depending on your location.

About Colorado

Colorado is located southwestern part of the United States, encompassing most of the southern Rocky Mountains, as well as the western edge of the Great Plains. It is the eighth largest state, in the country, and ranks twenty-first in population size. Colorado is also known as the "Centennial State" as it became a state one hundred years after the signing of the Declaration of Independence.


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