Colorado Drug Testing


Our testing locations do not accept payment and require a drug test order form at the time of testing. Please click the "Order a Test" button to order your test. Once your order has been processed, your drug test order form will be emailed to you.


Colorado Drug And Alcohol Testing

The state of Colorado, like many in the United States, is struggling with the influx of illicit substances and the impact of drug and alcohol use. In 2015, Colorado was ranked the highest in the country for use of opioid painkillers, alcohol, cocaine and marijuana.

Additionally, in 2015 there were more deaths in Colorado as a result of drug and alcohol abuse than by car crashes. The rate of fatalities from opioid overdoses increased by more than a multiple of three between 2000 and 2015.

Drug Testing Facilities

US Drug Test Centers is proud to offer 370 immediate drug and alcohol testing locations in the state of Colorado. Some of our collection sites are available for same-day drug and alcohol screenings and walk-ins, including Alamosa, Aurora, and several other major cities in the state of Colorado. To schedule, simply call 1-866-566-0261 before 10 am on the morning of your test. Walk-in rapid drug and alcohol screening test collection services are also available at some of our testing centers.

For full-service drug testing in Colorado, contact US Drug Test Centers today. We offer pre-employment testing, DOT drug and alcohol testing, Non-DOT testing, random drug testing, reasonable suspicion and post-accident testing. Additionally, US Drug Test Centers also offers instant drug testing with same-day results.

See below for a full list of testing centers in Colorado.

US Drug Test Centers

US Drug Test Centers is the complete, drug-free workplace solution for employers. We provide drug testing for all reasons such as pre-employment, random, reasonable suspicion, post-accident, and return to duty.We have a state-of-the-art cloud-based software that allows you to order drug testing 24/7, store all drug test results and chain of custody forms (forever), and you can see the status of all completed drug testing.

We have random drug and alcohol testing consortiums and follow all DOT rules and regulations to direct your company in the right direction to stay in compliance. At USDTC, we can create drug-free workplace policy manuals specific to your company’s needs, and we provide online trainings.

Our online trainings are perfect for company Supervisors to learn what signs to look for if someone might be under the influence. We also have trainings that explain what Supervisors should know about the DOT rules and regulations in regards to the drug and alcohol testing program.

We are a part of the Drug and Alcohol Testing Industry Association (DATIA), and we always stay up to date with all drug testing-related rules and regulations. If you have any questions about state-specific laws regarding drug and alcohol testing, please contact our office and one of our team members will be glad to help you. If your company would like more information regarding setting up a corporate account you can set up an account online or contact us at 866-566-0261.

369 Testing locations in Colorado

6609 County Road 114 South

Phone: 866-566-0261

5511 West 56th Ave

Phone: 866-566-0261

6140 S Gun Club Rd

Phone: 866-566-0261

6140 K6 South Gun Club Road #2

Phone: 866-566-0261

15235 East 38th Avenue

Phone: 866-566-0261

10355 E. Iliff Avenue

Phone: 866-566-0261

3449 N Chambers Rd

Phone: 866-566-0261

3797 Windsor Drive

Phone: 866-566-0261

14097 East Exposition Ave.

Phone: 866-566-0261

1444 South Potomac Street

Phone: 866-566-0261

1550 South Potomac Street

Phone: 866-566-0261

1550 S Potomac, Ste 325

Phone: 866-566-0261

16455 East 40th Circle

Phone: 866-566-0261

1250 South Buckley Road

Phone: 866-566-0261

1411 S Potomac St

Phone: 866-566-0261

14100 E Jewell Ave

Phone: 866-566-0261

14100 E. Jewell Ave

Phone: 866-566-0261

13650 East Mississippi Avenue

Phone: 866-566-0261

18121 East Hampden Avenue

Phone: 866-566-0261

2260 South Xanadu Way

Phone: 866-566-0261

1701 Chambers Rd

Phone: 866-566-0261

2323 S Troy St Bldg 1

Phone: 866-566-0261

1701 Chambers Road

Phone: 866-566-0261

142 Beaver Creek Place

Phone: 866-566-0261

230 Chapel Place

Phone: 866-566-0261

1000 Alpine Ave Ste 160

Phone: 866-566-0261

3300 28th Street

Phone: 866-566-0261

5377 Manhattan Circle #100

Phone: 866-566-0261

5387 Manhattan Cir

Phone: 866-566-0261

4800 Baseline Road

Phone: 866-566-0261

1690 30th Street

Phone: 866-566-0261

400 North Park Avenue

Phone: 866-566-0261

12060 Sable Blvd. #1

Phone: 866-566-0261

12060 Sable Blvd. Suite 1

Phone: 866-566-0261

275-c Pavilion Place

Phone: 866-566-0261

2801 Purcell Street

Phone: 866-566-0261

899 Us Highway 287 Ste 500

Phone: 866-566-0261

7000 West 117th Avenue

Phone: 866-566-0261

2400 W. Edison Street

Phone: 866-566-0261

2400 Edison Street

Phone: 866-566-0261

28374 County Rd 317

Phone: 866-566-0261

49801 Us Highway 24

Phone: 866-566-0261

3239 Independence Rd.

Phone: 866-566-0261

6093 South Quebec Street

Phone: 866-566-0261

11877 East Arapahoe Road

Phone: 866-566-0261

7384 South Alton Way

Phone: 866-566-0261

14100 East Arapahoe Road

Phone: 866-566-0261

8200 South Quebec Street

Phone: 866-566-0261

20971 E Smoky Hill Rd

Phone: 866-566-0261

20971 E. Smokey Hill Rd. 105 B

Phone: 866-566-0261

2305 E Arapahoe Rd

Phone: 866-566-0261

2305 E Arapahoe Rd Ste 147

Phone: 866-566-0261

724 South Tejon Street

Phone: 866-566-0261

3010 North Circle Drive

Phone: 866-566-0261

6011 East Woodmen Road

Phone: 866-566-0261

3030 N. Cir. Dr., Ste. 210

Phone: 866-566-0261

327 E. Pikes Peak Avenue

Phone: 866-566-0261

1633 Medical Center Point

Phone: 866-566-0261

5320 Mark Dabling Blvd.

Phone: 866-566-0261

5320 Mark Dabling Blvd

Phone: 866-566-0261

3366 Templeton Gap Road

Phone: 866-566-0261

4083 Austin Bluffs Pkwy

Phone: 866-566-0261

1495 Garden Of The Gods Road West

Phone: 866-566-0261

1495 Garden Of The Gods Rd

Phone: 866-566-0261

1035 West Garden Of The Gods Road

Phone: 866-566-0261

4323 Integrity Center Point

Phone: 866-566-0261

3225 International Cir,ste 200

Phone: 866-566-0261

175 South Union Boulevard

Phone: 866-566-0261

3205 Academy Boulevard North

Phone: 866-566-0261

2620 Tenderfoot Hill St

Phone: 866-566-0261

5101 N Quebec Street

Phone: 866-566-0261

1011 North Mildred Road

Phone: 866-566-0261

343 E. Main Street

Phone: 866-566-0261

641 East Main Street

Phone: 866-566-0261

301 East Main Street

Phone: 866-566-0261

33 N Chestnut Street

Phone: 866-566-0261

333 E Victory Way

Phone: 866-566-0261

750 Hospital Loop

Phone: 866-566-0261

600 Russell Street

Phone: 866-566-0261

595 Russell Street

Phone: 866-566-0261

209 4th Street, Suite A-2

Phone: 866-566-0261

233 Cottonwood St

Phone: 866-566-0261

9725 East Hampden Avenue Ste. 204

Phone: 866-566-0261

4600 South Syracuse Street

Phone: 866-566-0261

4600 S Syracuse St.

Phone: 866-566-0261

6240 Federal Blvd

Phone: 866-566-0261

3401 Quebec Street

Phone: 866-566-0261

6900 E 47th Ave Dr Ste 100

Phone: 866-566-0261

4705 East 48th Avenue

Phone: 866-566-0261

420 East 58th Sreet

Phone: 866-566-0261

5855 Stapleton Drive North

Phone: 866-566-0261

875 S Colorado Blvd.

Phone: 866-566-0261

3600 E Alameda Ave

Phone: 866-566-0261

1212 S. Broadway, Ste. 150

Phone: 866-566-0261

5855 E Stapleton Dr N

Phone: 866-566-0261

8700 Pena Boulevard

Phone: 866-566-0261

3700 Havana Street

Phone: 866-566-0261

7120 E. 49th Ave

Phone: 866-566-0261

51 West 84th Avenue

Phone: 866-566-0261

1780 South Bellaire Street

Phone: 866-566-0261

1570 Humboldt St Fl 1

Phone: 866-566-0261

8333 Greenwood Blvd, Suite 215

Phone: 866-566-0261

6900 East 47th Avenue

Phone: 866-566-0261

1515 Wazee St.,ste D

Phone: 866-566-0261

5855 Stapleton Dr N Ste A130

Phone: 866-566-0261

1730 Blake St Ste 200

Phone: 866-566-0261

5700 Lincoln Drive

Phone: 866-566-0261

4704 Harlan St Ste 100

Phone: 866-566-0261

120 Bryant Street

Phone: 866-566-0261

9501 Northfield Blvd

Phone: 866-566-0261

4500 Cherry Creek Drive South

Phone: 866-566-0261

2420 W. 26th Ave Bldg. D

Phone: 866-566-0261

605 Bannock Street

Phone: 866-566-0261

1405 Curtis Street

Phone: 866-566-0261

9501 Northfield Boulevard

Phone: 866-566-0261

1385 S Colorado Blvd

Phone: 866-566-0261

6795 E Tennessee Ave

Phone: 866-566-0261

810 East 3rd Street

Phone: 866-566-0261

450 South Camino Del Rio

Phone: 866-566-0261

3473 North Main Ave.

Phone: 866-566-0261

1050 Avenida Del Sol

Phone: 866-566-0261

1010 3 Springs Boulevard

Phone: 866-566-0261

377 Sylvan Lake Road, #210

Phone: 866-566-0261

322 Beard Creek Road

Phone: 866-566-0261

56 Edwards Village Blvd

Phone: 866-566-0261

12150 E. Briarwood Avenue

Phone: 866-566-0261

125 West Hampden Avenue

Phone: 866-566-0261

125 E. Hampden Ave

Phone: 866-566-0261

499 E Hampden Ave Ste 290

Phone: 866-566-0261

730 W Hampden Ave Ste 200

Phone: 866-566-0261

2425 I25 Frontage Rd

Phone: 866-566-0261

131 Stanley Ave Ste 202

Phone: 866-566-0261

28577 Buffalo Park Road

Phone: 866-566-0261

620 South Lemay Avenue

Phone: 866-566-0261

2620 E Prospect Rd Ste 160

Phone: 866-566-0261

1414 E. Harmony Rd. Suite 5

Phone: 866-566-0261

1040 East Elizabeth Street

Phone: 866-566-0261

2126 Milestone Dr, Ste 121

Phone: 866-566-0261

4674 Snow Mesa Drive

Phone: 866-566-0261

1600 Specht Point Road

Phone: 866-566-0261

112 South Denver Avenue

Phone: 866-566-0261

1310 Factory Circle

Phone: 866-566-0261

909 East Railroad Avenue

Phone: 866-566-0261

219 East Railroad Avenue

Phone: 866-566-0261

900 Skinny Camp Rd

Phone: 866-566-0261

340 Peak One Dr.

Phone: 866-566-0261

340 Peak One Drive

Phone: 866-566-0261

Highway 9 And School Road

Phone: 866-566-0261

770 Simms St Ste 100

Phone: 866-566-0261

1120 Wellington Ave, Ste 208

Phone: 866-566-0261

1236 North 7th Street

Phone: 866-566-0261

3150 North 12th Street

Phone: 866-566-0261

1517 16th Avenue Court

Phone: 866-566-0261

1900 16th Street

Phone: 866-566-0261

3510-b W. 10th St.

Phone: 866-566-0261

4663 West 20th Street

Phone: 866-566-0261

7257 West 4th Street

Phone: 866-566-0261

7251 West 20th Street

Phone: 866-566-0261

822 7th St Plaza 10

Phone: 866-566-0261

2528 West 16th Street

Phone: 866-566-0261

5650 Greenwood Pl Blvd.

Phone: 866-566-0261

8200 East Belleview Avenue

Phone: 866-566-0261

711 North Taylor Street

Phone: 866-566-0261

410 Mcgregor Drive

Phone: 866-566-0261

11750 East 124th Avenue

Phone: 866-566-0261

9330 S University Blvd

Phone: 866-566-0261

206 W County Line Rd Ste 150

Phone: 866-566-0261

1001 E. Johnson St

Phone: 866-566-0261

22449 County Road 35

Phone: 866-566-0261

14324 Highway 172

Phone: 866-566-0261

1100 Carson Avenue

Phone: 866-566-0261

1200 West South Boulder Road

Phone: 866-566-0261

1000 W South Boulder Rd

Phone: 866-566-0261

1201 E South Boulder Rd

Phone: 866-566-0261

11185 West 6th Avenue

Phone: 866-566-0261

12790 W. Alameda Pkwy

Phone: 866-566-0261

137 Union Boulevard

Phone: 866-566-0261

3900 South Wadsworth Boulevard

Phone: 866-566-0261

7050 West Hampden Avenue

Phone: 866-566-0261

7400 W. 14th Ave.

Phone: 866-566-0261

200 Union Blvd Ste 318

Phone: 866-566-0261

7585 W Arkansas Ave

Phone: 866-566-0261

7585 W Arkansas #b

Phone: 866-566-0261

306 South 5th Street

Phone: 866-566-0261

20 W. Dry Creek Circle

Phone: 866-566-0261

6169 S Balsam Way Ste 150

Phone: 866-566-0261

1551 Professional Lane

Phone: 866-566-0261

1020 Ken Pratt Boulevard

Phone: 866-566-0261

1925 W. Mountain View Ave

Phone: 866-566-0261

1950 Mountainview Ave

Phone: 866-566-0261

1900 South Sunset Street

Phone: 866-566-0261

2130 Mountain View Ave

Phone: 866-566-0261

1860 Industrial Circle

Phone: 866-566-0261

205 South Main Street

Phone: 866-566-0261

2529 N Lincoln Ave

Phone: 866-566-0261

1703 East 18th Street

Phone: 866-566-0261

4196 North Garfield Avenue

Phone: 866-566-0261

4196 N. Garfield Ave

Phone: 866-566-0261

2529 N. Lincoln Ave Suite C

Phone: 866-566-0261

345 Cleveland Street

Phone: 866-566-0261

2305 S. Townsend Ave.

Phone: 866-566-0261

816 South 5th Street

Phone: 866-566-0261

1329 South Townsend Avenue

Phone: 866-566-0261

10699 Melody Drive

Phone: 866-566-0261

100 East 120th Avenue

Phone: 866-566-0261

95 South Pagosa Boulevard

Phone: 866-566-0261

1482 Highway 133 Ste 7

Phone: 866-566-0261

71 Sipprelle Drive

Phone: 866-566-0261

4112 Outlook Blvd Ste 37

Phone: 866-566-0261

4117 N Elizabeth St

Phone: 866-566-0261

1619 North Greenwood Street

Phone: 866-566-0261

1306 N Grand Ave

Phone: 866-566-0261

56 Club Manor Dr Ste 102

Phone: 866-566-0261

2025 North Norwood Ave, Suite

Phone: 866-566-0261

2025 North Norwood Ave. Ste. B

Phone: 866-566-0261

3676 Parker Blvd

Phone: 866-566-0261

503 Avocado Street

Phone: 866-566-0261

321 East Main Street, #2

Phone: 866-566-0261

511 S. White Avenue

Phone: 866-566-0261

300 Railroad Ave

Phone: 866-566-0261

3921 County Road 233

Phone: 866-566-0261

129 West 4th Street

Phone: 866-566-0261

3161 Baron Lane Unit E

Phone: 866-566-0261

1530 Railroad Ave

Phone: 866-566-0261

1530 Railroad Ave Ste A

Phone: 866-566-0261

2001 Horseshoe Trail

Phone: 866-566-0261

373 E. Tenth Avenue

Phone: 866-566-0261

1475 Pine Grove Rd Ste 102

Phone: 866-566-0261

1024 Central Park Drive

Phone: 866-566-0261

3001 South Lincoln Avenue

Phone: 866-566-0261

375 Charmony Frontage Rd

Phone: 866-566-0261

550 Thornton Parkway

Phone: 866-566-0261

500 E 84th Ave Ste B14

Phone: 866-566-0261

9195 Grant Street #100

Phone: 866-566-0261

181 W Meadow Dr

Phone: 866-566-0261

181 W Meadow Dr Ste 100

Phone: 866-566-0261

8510 North Bryant Street

Phone: 866-566-0261

1011 Baltimore Blvd

Phone: 866-566-0261

12207 Pecos St.,ste 300

Phone: 866-566-0261

12213 Pecos St #600

Phone: 866-566-0261

12151 West 44th Avenue

Phone: 866-566-0261

4980 Kipling Street

Phone: 866-566-0261

9830 West I-70 Frontage Rd So

Phone: 866-566-0261

9299 Eastman Park Dr

Phone: 866-566-0261

15 East Walnut St

Phone: 866-566-0261

1017 W. 7th St.

Phone: 866-566-0261

Marijuana Use in a Legalized State

Countless employers in Colorado are implementing drug testing programs, during the hiring process, to filter our potential employees who smoke marijuana. Although medical marijuana and recreational marijuana are legal in Colorado, employers have every right to implement and maintain a drug-free workplace program, which includes testing for marijuana (medical or otherwise). Recent studies show an increase in the number of employers who have established drug testing programs in the workplace since recreational marijuana use was legalized in 2014.

The Colorado Supreme Court has issued a ruling on the topic of medical marijuana and employment practices. In Coats v. Dish Network, an employee sued for wrongful termination after he tested positive for marijuana. The plaintiff is a quadriplegic who used medical marijuana outside of working hours. Mr. Coats was a registered medical marijuana user, accessing the product in a manner consistent with state constitutional guarantees and state statute. The use of marijuana is still illegal under Federal law. Dish Network has a comprehensive drug free workplace policy. The court held in a unanimous opinion that the term “lawful activity” extends to both state and federal law.

According to Federal law, marijuana is a schedule I drug. Under that classification, marijuana “has a high potential for abuse…has no currently accepted medical use in treatment in the United States [and] there is a lack of accepted safety for use of the drug or other substance under medical supervision” [21 U.S.C 812(b)(1)].

Employers in the state of Colorado have a right to implement a employee drug testing program. The state of Colorado imposes no restrictions except in the City of Boulder. US Drug Test Centers offers both DOT and non-DOT drug testing programs for employers in Colorado. Setting up a workplace program is simple and affordable with your testing specialists from US Drug Test Centers.

Why Choose US Drug Test Centers

There are various reasons why you may have a need for a drug or alcohol test. US Drug Test Centers offers drug testing for individuals with same day service. Drug testing is available for personal reasons, probation drug testing, DUI drug testingchild custody cases, divorce cases and court-ordered programs. US Drug Test Centers also works with Colorado-based attorneys in setting up drug or alcohol testing for their clients. In many cases, same day drug test results are available on request.

US Drug Test Centers drug testing collection sites are available in all areas of Colorado including the City of Colorado Spring, Denver, City of Aurora, City of Fort Collins, City of Grand Junction, City of Lakewood, City of Thornton, City of Pueblo, City of Arvada, City of Westminster, City of Centennial, City of Boulder, City of Greeley, Town of Castle Rock, City of Longmont, City of Loveland,, City and County of Broomfield,, City of Commerce City, City of Englewood, Town of Parker City of Littleton, City of Northglenn, City of Brighton, City of Wheat Ridge, City of Fountain and the City of Lafayette.

Call US Drug Test Centers at 866-566-0261 today for drug and alcohol, hair testing and EtG alcohol testing. Testing options and additional fees may vary depending on your location.

About Colorado

Colorado is located southwestern part of the United States, encompassing most of the southern Rocky Mountains, as well as the western edge of the Great Plains. It is the eighth largest state, in the country, and ranks twenty-first in population size. Colorado is also known as the "Centennial State" as it became a state one hundred years after the signing of the Declaration of Independence.

Testing options may vary on a site by site basis. Additional fees may apply depending on your location.

Frequently Asked Questions

This material is intended for informational purposes only and should not be relied upon for legal guidance. State & local laws change frequently and vary greatly. These are state & local laws and do not over rule the federal Department of Transportation laws & policies. Readers are advised to consult with their legal counsel or industry experts before acting based on this information. Last Updated June 2019

Colorado Drug Testing FAQs

No.  Colorado does not have any mandatory drug testing statutes or regulations. However, employers in the city of Boulder, CO face stringent drug testing rules, due to a city ordinance.
No, however, employers are strongly encouraged to have a written policy in place (Policy is specifically required to comply with Colorado’s voluntary law).
Yes. Employers may leverage a ‘Rebuttable Presumption of Intoxication’ defense to a Workers’ Compensation Claim, based on a positive drug test result to qualify for a 50% in benefit award. A positive drug test may also make an employee ineligible for unemployment compensation. Employers wishing to leverage either of these benefits must voluntarily comply with very detailed program rules.
Yes. Colorado permits the personal adult use and the medical use of marijuana for patients that meet specific medical conditions. The laws do not specifically impact employment drug testing programs.